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magnetic labeling with anti cd1a  (Miltenyi Biotec)


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    Miltenyi Biotec magnetic labeling with anti cd1a
    Magnetic Labeling With Anti Cd1a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd1a+microbeads/CD1a+MicroBeads%2C+human/bio_rxiv__64898__2026__05__05__723095-237-13-20
    Average 93 stars, based on 42 article reviews
    magnetic labeling with anti cd1a - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Purification:

    Article Title: Autophagy-enhancing drugs limit mucosal HIV-1 acquisition and suppress viral replication ex vivo.
    Article Snippet: .. Further vaginal LC purification was performed using a Ficoll gradient and CD1a microbeads (Miltenyi Biotec). ..

    Article Title: HIV-1 subverts the complement system in semen to enhance viral transmission.
    Article Snippet: .. Mucosal Immunology (2021) 14:743 – 750 gradient (Axis-shield) and immature LCs were purified using CD1a microbeads (Miltenyi Biotec, Germany). ..

    Labeling:

    Article Title: A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals
    Article Snippet: .. On day 6, CD1a + MoLCs were magnetically labeled and isolated using CD1a MicroBeads, human (Miltenyi Biotech, Bergisch Gladbach, Germany). .. CD4 + naïve T-lymphocytes were isolated from PBMC using the naive CD4 + T-Cell Isolation Kit II, human, as recommended by the manufacturer (Miltenyi Biotech, Bergisch-Gladbach, Germany).

    Isolation:

    Article Title: A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals
    Article Snippet: .. On day 6, CD1a + MoLCs were magnetically labeled and isolated using CD1a MicroBeads, human (Miltenyi Biotech, Bergisch Gladbach, Germany). .. CD4 + naïve T-lymphocytes were isolated from PBMC using the naive CD4 + T-Cell Isolation Kit II, human, as recommended by the manufacturer (Miltenyi Biotech, Bergisch-Gladbach, Germany).

    Suspension:

    Article Title: Coencapsulation of Immunosuppressive Drug with Anti-Inflammatory Molecule in Pickering Emulsions as an Innovative Therapeutic Approach for Inflammatory Dermatoses
    Article Snippet: .. The epidermal cell suspension was then filtered through a 30-μm cell strainer, counted, and either directly seeded in 100 mm 2 petri dish to culture keratinocyte in Keratinocyte Growth Medium or used to purify LCs by positive selection using the CD1a microbeads (Miltenyi Biotec), according to the manufacturer’s instructions. ..

    Selection:

    Article Title: Coencapsulation of Immunosuppressive Drug with Anti-Inflammatory Molecule in Pickering Emulsions as an Innovative Therapeutic Approach for Inflammatory Dermatoses
    Article Snippet: .. The epidermal cell suspension was then filtered through a 30-μm cell strainer, counted, and either directly seeded in 100 mm 2 petri dish to culture keratinocyte in Keratinocyte Growth Medium or used to purify LCs by positive selection using the CD1a microbeads (Miltenyi Biotec), according to the manufacturer’s instructions. ..

    Article Title: Usutu Virus escapes langerin-induced restriction to productively infect human Langerhans cells, unlike West Nile virus
    Article Snippet: Epidermal sheets were cultured separately in RPMI with 10% human AB serum (Thermo Fisher Scientific) and 1% Penicillin/Streptomycin/Amphotericin B solution for 48 h, after which migratory cells were collected from media. .. Migratory cells were either used directly for experiments or subjected to a positive selection using CD1a Microbeads (Miltenyi Biotec), in order to enrich epidermal Langerhans cells. ..



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    Image Search Results


    Characterization of ImmuSkin-MT, ImmuSkin-M, and ImmuSkin-T. Skin models were subjected to H&E and immunofluorescence staining against loricrin as a marker for differentiated keratinocytes (red) and CD1a as a specific DC marker (green). DAPI (blue) was used as a nucleus counter-staining. Carboxyfluorescein succinimidyl ester (CFSE, green) is an indicator of T-lymphocytes indicated the absence of T-cells in the epidermis, dermis, and the immune layer. The white dotted line indicates the boundary between epidermis and dermis. In some pictures from immunofluorescence staining, a membrane of the transwell insert is presented in a bright yellow-green strip. Images were captured at a magnification of 20x. Scale bar = 100 µm. n = 3. The schematic part of this figure was created with BioRender.com

    Journal: Archives of Toxicology

    Article Title: A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals

    doi: 10.1007/s00204-025-04130-z

    Figure Lengend Snippet: Characterization of ImmuSkin-MT, ImmuSkin-M, and ImmuSkin-T. Skin models were subjected to H&E and immunofluorescence staining against loricrin as a marker for differentiated keratinocytes (red) and CD1a as a specific DC marker (green). DAPI (blue) was used as a nucleus counter-staining. Carboxyfluorescein succinimidyl ester (CFSE, green) is an indicator of T-lymphocytes indicated the absence of T-cells in the epidermis, dermis, and the immune layer. The white dotted line indicates the boundary between epidermis and dermis. In some pictures from immunofluorescence staining, a membrane of the transwell insert is presented in a bright yellow-green strip. Images were captured at a magnification of 20x. Scale bar = 100 µm. n = 3. The schematic part of this figure was created with BioRender.com

    Article Snippet: On day 6, CD1a + MoLCs were magnetically labeled and isolated using CD1a MicroBeads, human (Miltenyi Biotech, Bergisch Gladbach, Germany).

    Techniques: Immunofluorescence, Staining, Marker, Membrane, Stripping Membranes

    Influence of cocultivation on the migration and maturation of MoLCs and the proliferation of T-cells. Cells harvested from the lower chamber of each ImmuSkin variant were subjected to flow cytometry analysis and stained with anti-CD1a-, anti-CD86- and anti CD4-antibodies. a Representative dot plots show the populations of T-lymphocytes and MoLCs from each ImmuSkin type. The upper left side of the quadrant represents T-lymphocytes, while MoLCs are shown in the lower right side of the quadrant. Moreover, MoLC migration ( b ), MoLC maturation ( c ) and T-cell proliferation ( d ) were measured. The black bar represents immune cells from ImmuSkin-M, the striped bar represents immune cells from ImmuSkin-T, while the gray bar represents immune cells from ImmuSkin-MT. Data are presented as mean ± SD, and statistical significance is indicated as * p -value ≤ 0.05, ** p -value ≤ 0.01, *** p -value ≤ 0.001. A paired t-test was used for the statistical analysis. n = 3. The schematic part of this figure was created with BioRender.com

    Journal: Archives of Toxicology

    Article Title: A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals

    doi: 10.1007/s00204-025-04130-z

    Figure Lengend Snippet: Influence of cocultivation on the migration and maturation of MoLCs and the proliferation of T-cells. Cells harvested from the lower chamber of each ImmuSkin variant were subjected to flow cytometry analysis and stained with anti-CD1a-, anti-CD86- and anti CD4-antibodies. a Representative dot plots show the populations of T-lymphocytes and MoLCs from each ImmuSkin type. The upper left side of the quadrant represents T-lymphocytes, while MoLCs are shown in the lower right side of the quadrant. Moreover, MoLC migration ( b ), MoLC maturation ( c ) and T-cell proliferation ( d ) were measured. The black bar represents immune cells from ImmuSkin-M, the striped bar represents immune cells from ImmuSkin-T, while the gray bar represents immune cells from ImmuSkin-MT. Data are presented as mean ± SD, and statistical significance is indicated as * p -value ≤ 0.05, ** p -value ≤ 0.01, *** p -value ≤ 0.001. A paired t-test was used for the statistical analysis. n = 3. The schematic part of this figure was created with BioRender.com

    Article Snippet: On day 6, CD1a + MoLCs were magnetically labeled and isolated using CD1a MicroBeads, human (Miltenyi Biotech, Bergisch Gladbach, Germany).

    Techniques: Migration, Variant Assay, Flow Cytometry, Staining